The significant volume of bio-waste is coming from horticulture due to the treatment of perennial plantations. Horticulture wooden waste looks like a promising feedstock for the further utilization by bio-based energy sector. Moreover, using waste obtained in the result of-trees’ trimming and perforation might provide high economic and environmental impact in the energy balance of the country. Unfortunately, currently, the potential of this wooden waste is not used fully. Partly, this caused with lack of accurate data about available resource especially in the developing countries such as Ukraine. In this study, assessment of energy, economic and geographical potential of horticulture wooden waste was proposed following up to case-study of biomass potential analysis. The research was based on the case study in one region but the obtained results were scaled on the country level. Additionally, it was found that the diversity of methods of waste biomass volume assessment in horticulture depended on the farm type, since highly developed industrial farms and small households carry out gardening on the different ways.